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Miniaturization of cultures and fatty acid analysis for the characterization of elongases. A Experimental steps for entire workflow were adapted to be performed entirely <t>in</t> <t>96-well</t> format. B Comparison of fatty acid profiles obtained from strains expressing elongases between the standard (Std.) and the miniaturized (Mini.) protocols. Fatty acid content of strains was analyzed by GC-FID/MS. The concentration of each fatty acid was reported relative to the total fatty acid concentration. C Fatty acid concentrations expressed in g/L. The data represent the mean ± standard deviation ( n ≥ 3)
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Miniaturization of cultures and fatty acid analysis for the characterization of elongases. A Experimental steps for entire workflow were adapted to be performed entirely <t>in</t> <t>96-well</t> format. B Comparison of fatty acid profiles obtained from strains expressing elongases between the standard (Std.) and the miniaturized (Mini.) protocols. Fatty acid content of strains was analyzed by GC-FID/MS. The concentration of each fatty acid was reported relative to the total fatty acid concentration. C Fatty acid concentrations expressed in g/L. The data represent the mean ± standard deviation ( n ≥ 3)
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nunc 96  (Thermo Fisher)
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Miniaturization of cultures and fatty acid analysis for the characterization of elongases. A Experimental steps for entire workflow were adapted to be performed entirely <t>in</t> <t>96-well</t> format. B Comparison of fatty acid profiles obtained from strains expressing elongases between the standard (Std.) and the miniaturized (Mini.) protocols. Fatty acid content of strains was analyzed by GC-FID/MS. The concentration of each fatty acid was reported relative to the total fatty acid concentration. C Fatty acid concentrations expressed in g/L. The data represent the mean ± standard deviation ( n ≥ 3)
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Miniaturization of cultures and fatty acid analysis for the characterization of elongases. A Experimental steps for entire workflow were adapted to be performed entirely <t>in</t> <t>96-well</t> format. B Comparison of fatty acid profiles obtained from strains expressing elongases between the standard (Std.) and the miniaturized (Mini.) protocols. Fatty acid content of strains was analyzed by GC-FID/MS. The concentration of each fatty acid was reported relative to the total fatty acid concentration. C Fatty acid concentrations expressed in g/L. The data represent the mean ± standard deviation ( n ≥ 3)
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Thermo Fisher nunc tm deepwell plates
Miniaturization of cultures and fatty acid analysis for the characterization of elongases. A Experimental steps for entire workflow were adapted to be performed entirely <t>in</t> <t>96-well</t> format. B Comparison of fatty acid profiles obtained from strains expressing elongases between the standard (Std.) and the miniaturized (Mini.) protocols. Fatty acid content of strains was analyzed by GC-FID/MS. The concentration of each fatty acid was reported relative to the total fatty acid concentration. C Fatty acid concentrations expressed in g/L. The data represent the mean ± standard deviation ( n ≥ 3)
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96 deep well plates  (Thermo Fisher)
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Thermo Fisher 96 deep well plates
Miniaturization of cultures and fatty acid analysis for the characterization of elongases. A Experimental steps for entire workflow were adapted to be performed entirely <t>in</t> <t>96-well</t> format. B Comparison of fatty acid profiles obtained from strains expressing elongases between the standard (Std.) and the miniaturized (Mini.) protocols. Fatty acid content of strains was analyzed by GC-FID/MS. The concentration of each fatty acid was reported relative to the total fatty acid concentration. C Fatty acid concentrations expressed in g/L. The data represent the mean ± standard deviation ( n ≥ 3)
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96 well midi plate  (Thermo Fisher)
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Miniaturization of cultures and fatty acid analysis for the characterization of elongases. A Experimental steps for entire workflow were adapted to be performed entirely <t>in</t> <t>96-well</t> format. B Comparison of fatty acid profiles obtained from strains expressing elongases between the standard (Std.) and the miniaturized (Mini.) protocols. Fatty acid content of strains was analyzed by GC-FID/MS. The concentration of each fatty acid was reported relative to the total fatty acid concentration. C Fatty acid concentrations expressed in g/L. The data represent the mean ± standard deviation ( n ≥ 3)
96 Well Midi Plate, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Miniaturization of cultures and fatty acid analysis for the characterization of elongases. A Experimental steps for entire workflow were adapted to be performed entirely in 96-well format. B Comparison of fatty acid profiles obtained from strains expressing elongases between the standard (Std.) and the miniaturized (Mini.) protocols. Fatty acid content of strains was analyzed by GC-FID/MS. The concentration of each fatty acid was reported relative to the total fatty acid concentration. C Fatty acid concentrations expressed in g/L. The data represent the mean ± standard deviation ( n ≥ 3)

Journal: Microbial Cell Factories

Article Title: A workflow to explore elongase diversity and extend the repertoire of fatty acids produced by Yarrowia lipolytica

doi: 10.1186/s12934-025-02890-y

Figure Lengend Snippet: Miniaturization of cultures and fatty acid analysis for the characterization of elongases. A Experimental steps for entire workflow were adapted to be performed entirely in 96-well format. B Comparison of fatty acid profiles obtained from strains expressing elongases between the standard (Std.) and the miniaturized (Mini.) protocols. Fatty acid content of strains was analyzed by GC-FID/MS. The concentration of each fatty acid was reported relative to the total fatty acid concentration. C Fatty acid concentrations expressed in g/L. The data represent the mean ± standard deviation ( n ≥ 3)

Article Snippet: Cultures were then inoculated at OD 0.5 into 96-well deepwell plates (AB-0661, ABgene, Thermo Scientific) filled with 500 μL of YT 2 D 5 .

Techniques: Comparison, Expressing, Concentration Assay, Standard Deviation